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    Rabbit Anti-CD19 antibody (bs-0079R)
    ~~~促銷,代碼KT22301~~~
    ~~~促銷,代碼KT22302~~~
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    產品編號 bs-0079R
    英文名稱 CD19
    中文名稱 CD19抗體
    別    名 CD19_HUMAN; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19; B-lymphocyte surface antigen B4; Leu 12; Leu12;   
    Specific References  (9)     |     bs-0079R has been referenced in 9 publications.
    [IF=2.245] Taotao Li. et al. Integrating miRNA and mRNA Profiling to Assess the Potential miRNA–mRNA Modules Linked With Testicular Immune Homeostasis in Sheep. Front Vet Sci. 2021; 8: 647153  WB,IF ;  Sheep.  
    [IF=4.344] Gitto SB et al. Identification of a novel IL-5 signaling pathway in chronic pancreatitis and crosstalk with pancreatic tumor cells. Cell Commun Signal. 2020 Jun 17;18(1):95.  IHC-P ;  Mouse.  
    [IF=3.943] Yihan Lu? et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563.  IF ;  Mouse.  
    [IF=2.515] Li T et al. Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis.R Soc Open Sci. 2019 Jul 3;6(7):181604.  IHC ;  Sheep.  
    [IF=4.1] Goodwin, Justin, et al. "Targeting HIF-1α/PDK1 Axis by Dichloroacetate (DCA) Suppresses Bleomycin-induced Pulmonary Fibrosis." American Journal of Respiratory Cell and Molecular Biology ja (2017).  IHC-P ;  Mouse.  
    [IF=0.52] Utomo, Pamudji, et al. "Decreasing SDF1-CXCR4 Expression after Adipose-Derived Mesenchymal Stem Cells (ASCS) Treatment Combined with Freeze-Dried Amniotic Membrane Wrapping in Rat Sciatic Nerve Injury." International Journal of ChemTech Research.  IF(ICC) ;  Rat.  
    [IF=1.813] Othman Hemn. et al. Antileukemic Effect of Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract In Vitro and in WEHI-3B-Induced Leukemia In Vivo. Evid-Based Compl Alt. 2020;2020:8764096  IHC ;  Mouse.  
    [IF=2.08] Takahashi, Yuichi, Yohei Ikezumi, and Akihiko Saitoh. "Rituximab protects podocytes and exerts anti‐proteinuric effects in rat adriamycin‐induced nephropathy independent of B‐lymphocytes." Nephrology (2016).  Rat.  
    [IF=3.54] Reese, Shannon R., et al. "Calcineurin Inhibitor Minimization With Ixazomib, an Investigational Proteasome Inhibitor, for the Prevention of Antibody Mediated Rejection in a Preclinical Model." Transplantation (2015).  IHC-P ;  Rat.  
    研究領域 細胞生物  免疫學  干細胞  細胞表面分子  淋巴細胞  b-淋巴細胞  
    抗體來源 Rabbit
    克隆類型 Polyclonal
    交叉反應 Human, Mouse, Rat,  (predicted: Pig, Cow, Horse, Guinea Pig, )
    產品應用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蠟切片需做抗原修復)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 59kDa
    檢測分子量 95 kDa
    細胞定位 細胞膜 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CD19: 485-556/556 <Cytoplasmic>
    亞    型 IgG
    純化方法 affinity purified by Protein A
    緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產品介紹 This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]

    Function:
    Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

    Subunit:
    Forms a complex with CD21, CD81 and CD225 in the membrane of mature B-cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Interacts with LYN.

    Subcellular Location:
    Membrane; Single-pass type I membrane protein.

    Post-translational modifications:
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN.

    DISEASE:
    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

    Similarity:
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    SWISS:
    P15391

    Gene ID:
    930

    Database links:

    Entrez Gene: 930 Human

    Entrez Gene: 12478 Mouse

    Entrez Gene: 365367 Rat

    Omim: 107265 Human

    SwissProt: P15391 Human

    SwissProt: P25918 Mouse

    Unigene: 652262 Human

    Unigene: 4360 Mouse



    CD19是一種質膜蛋白質,參與信號傳導作用。表達與前B細胞和成熟的B細胞膜表面,與B細胞的活化調節和發育調節相關,在T細胞和正常粒細胞上無表達。此抗體可以特異性識別CD19,主要用于標記正常B細胞及腫瘤性B細胞。
    產品圖片
    Tissue/cell: mouse fetal skin; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-CD19 Polyclonal Antibody, Unconjugated(bs-0079R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    Blank control (blue line): HL60 cells (blue).
    Primary Antibody (green line): Rabbit Anti-CD19 antibody (bs-0079R)
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    Blank control: Raji(blue).
    Primary Antibody: Rabbit Anti-CD19 antibody(bs-0079R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    Protocol
    Primary antibody (bs-0079R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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