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    產品編號 bs-0080R
    英文名稱 CD20
    中文名稱 CD20抗體
    別    名 CD20_HUMAN; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; MS4A1; MS4A2; B1; CVID5; LEU-16; S7.  
    Specific References  (2)     |     bs-0080R has been referenced in 2 publications.
    [IF=1.665] Ane M. Andres. et al. Graft infusion of adipose‐derived mesenchymal stromal cells to prevent rejection in experimental intestinal transplantation: A feasibility study. 2021 Feb 05  IHC ;  Rat.  
    [IF=3.514] Liu Y et al. Activation of the IL-6/JAK2/STAT3 pathway induces plasma cell mastitis in mice.Cytokine. 2018 Oct;110:150-158.  IHC-P ;  Mouse.  
    研究領域 腫瘤  免疫學  干細胞  細胞表面分子  細胞類型標志物  淋巴細胞  b-淋巴細胞  
    抗體來源 Rabbit
    克隆類型 Polyclonal
    交叉反應 Human, Mouse, Rat,  (predicted: Dog, Cow, Rabbit, )
    產品應用 WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=1μg /test (石蠟切片需做抗原修復)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 33kDa
    細胞定位 細胞膜 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CD20: 133-182/297 <Cytoplasmic>
    亞    型 IgG
    純化方法 affinity purified by Protein A
    緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產品介紹 CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.

    This protein may be involved in the regulation of B-cell activation and proliferation

    Subcellular Location:
    Membrane; Multi-pass membrane protein.

    Tissue Specificity:
    Expressed on B-cells.

    Post-translational modifications:
    Phosphorylated. Might be functionally regulated by protein kinase(s).

    Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

    Belongs to the MS4A family.


    Gene ID:

    Database links:

    Entrez Gene: 931 Human

    Omim: 112210 Human

    SwissProt: P11836 Human

    Unigene: 712553 Human

    [PHARMACEUTICAL] Monoclonal antibodies (mAb) against CD20 are used to treat B-cell non-Hodgkin lymphoma (NHL). These antibodies include Rituximab (Mabthera), Britumomab (Zevalin) and Tositumomab (Bexxar). CD20 engaged by mAb can generate transmembrane signals capable of directly controlling cell growth and triggering cell death in certain tumors. Alternatively, mAb can mediate complement-dependent cytotoxicity.


    Lane 1: Lymph node (Mouse) Lysate at 40 ug
    Lane 2: Bone (Mouse) Lysate at 40 ug
    Primary: Anti-CD20 (bs-0080R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 43 kD
    Observed band size: 43 kD
    Blank control: U937 (blue).
    Primary Antibody:Rabbit Anti- CD20 antibody(bs-0080R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-0080R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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