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    產品編號 bs-2938R
    英文名稱 DNTT
    中文名稱 末端脫氧核苷酸轉移酶抗體
    別    名 Deoxynucleotidyltransferase terminal; DNA nucleotidylexotransferase; TdT; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase; TDT_HUMAN.  
    研究領域 細胞生物  免疫學  染色質和核信號  
    抗體來源 Rabbit
    克隆類型 Polyclonal
    交叉反應 Human, Rat,  (predicted: Mouse, Dog, Horse, Rabbit, )
    產品應用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 (石蠟切片需做抗原修復)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 58kDa
    細胞定位 細胞核 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human TdT/DNTT: 351-450/509 
    亞    型 IgG
    純化方法 affinity purified by Protein A
    緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產品介紹 This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]

    Subunit:
    Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.

    Subcellular Location:
    Nucleus.

    Similarity:
    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.

    SWISS:
    P04053

    Gene ID:
    1791

    Database links:

    Entrez Gene: 1791 Human

    Entrez Gene: 21673 Mouse

    Entrez Gene: 294051 Rat

    Omim: 187410 Human

    SwissProt: P04053 Human

    SwissProt: P09838 Mouse

    Unigene: 534206 Human

    Unigene: 25620 Mouse

    Unigene: 143741 Rat



    產品圖片
    Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TdT) Polyclonal Antibody, Unconjugated (bs-2938R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
    Blank control (Black line):Molt4 (Black).
    Primary Antibody (green line): Rabbit Anti-TdT antibody (bs-2938R)
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    Molt-4 cells were fixed with 4% PFA for 10min at room temperature ,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TdT Antibody(bs-2938R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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