| 產品編號 | bs-10218R |
| 英文名稱 | CD5 |
| 中文名稱 | CD5抗體 |
| 別 名 | T-cell surface glycoprotein CD5; Lymphocyte antigen 1; Ly-1; Lyt-1; CD-5; CD5 antigen; CD 5; CD5 molecule; CD5 antigen (p56 62); CD5_HUMAN; LEU 1; LEU1; Ly12; LyA; Lymphocyte Antigen CD5; Lymphocyte antigen T1/Leu 1; Lymphocyte antigen T1/Leu-1; Lymphocyte glycoprotein T1/Leu1; OTTHUMP00000236973; p56 62; T1. |
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Specific References (1) | bs-10218R has been referenced in 1 publications.
[IF=2.751] Jiachen Lv. et al. Alcohol Aggravates Acute Pancreatitis by Impairing Autophagic Flux Through Activation of AMPK Signaling Pathway. 2021 Feb 08 IHC ; Human,Rat.
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| 研究領域 | 免疫學 干細胞 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Cow, Horse, Rabbit, Sheep, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD5: 221-320/495 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors. Function: May act as a receptor in regulating T-cell proliferation. Subunit: Interacts with CD72/LYB-2. Interacts with PTPN6/SHP-1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Post-translational modifications: Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1. Similarity: Contains 3 SRCR domains. SWISS: P06127 Gene ID: 921 Database links: Entrez Gene: 921 Human Omim: 153340 Human SwissProt: P06127 Human Unigene: 58685 Human
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| 產品圖片 |
Sample:
Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CD5 (bs-10218R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD 
Sample:
Jurkat(Human) Cell Lysate at 30 ug MOLT-4(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti-CD5 (bs-10218R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD 
Sample:
Thymus (Mouse) Lysate at 40 ug Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CD5 (bs-10218R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD 
Molt-4 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD5 Antibody(bs-10218R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
