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    Mouse Anti-CD10 antibody (bsm-51667M)
    ~~~促銷,代碼KT22301~~~
    ~~~促銷,代碼KT22302~~~
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    產品編號 bsm-51667M
    英文名稱 CD10
    中文名稱 CD10單克隆抗體
    別    名 NEP_HUMAN; Neprilysin; MME; membrane metalloendopeptidase; NEP; SFE; CD10; CALLA; CMT2T; SCA43; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; Neutral endopeptidase; Skin fibroblast elastase;   
    研究領域 腫瘤  細胞生物  免疫學  干細胞  細胞表面分子  細胞類型標志物  新陳代謝  
    抗體來源 Mouse
    克隆類型 Monoclonal
    克 隆 號 B4S1
    交叉反應 Human, 
    產品應用 WB=1:500-4000 IHC-P=1:100-400 IHC-F=1:100-400 Flow-Cyt=1:25 (石蠟切片需做抗原修復)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 82kDa
    細胞定位 細胞膜 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CD10: 401-550/750 <Extracellular>
    亞    型 IgG1,k
    純化方法 affinity purified by Protein G
    緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產品介紹 The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017]

    Function:
    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

    Subunit:
    Homotetramer.

    Subcellular Location:
    Cell membrane; Single-pass type II membrane protein.

    Post-translational modifications:
    Myristoylation is a determinant of membrane targeting.
    Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.

    DISEASE:
    Defects in GNMT are the cause of glycine N-methyltransferase deficiency (GNMT deficiency) [MIM:606664]; also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.

    Similarity:
    Belongs to the peptidase M13 family.

    SWISS:
    P08473

    Gene ID:
    4311

    Database links:

    Entrez Gene: 4311 Human

    SwissProt: P08473 Human



    產品圖片
    Sample:
    Lane 1: Human placenta tissue lysates
    Lane 2: Ramos cell lysates
    Lane 3: Human kidney tissue lysates
    Primary: Anti-CD10 (bsm-51667M) at 1/4000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 82 kD
    Observed band size:100 kD
    Blank control:Jurkat.
    Primary Antibody (green line): Mouse Anti-MME antibody (bsm-51667M)
    Dilution: 1:25;
    Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488
    Dilution: 1:400 Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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